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Targeted HMW DNA isolation kit for SageHLS. The SageHLS can process up to 2 samples per cassette and 2 cassettes per run.
The CATCH* process provides researchers with the most direct access to genes for study. Target regions are defined with the simple design of guide-RNAs to flank regions of interest — and enriched directly from cells. With intact full-length targets, genes can be deep sequenced with direct short read methods and/or analyzed with long range approaches.
Unravel difficult genomic targets
Some genes or genomic regions remain poorly characterized and are not easily analyzed by hybridization-based targeted sequencing or whole genome sequencing. The CATCH method allows researchers to use to zero in on these areas, many of which are implicated in cancers and inherited diseases.
Workflow
*Cas9-Assisted Targeting of CHromosomal segments
Kit content :
- Agarose gel cassettes RT
- Adhesive Tape Strips
- HLS Lysis Reagent 3% SDS
- HLS Enzyme Buffer
- Running Buffer
- 4X Enzyme Buffer (for Cas9-Guide RNA Mix)
- HLS Lysis Reagent 3% Sarkosyl
- HLS Lysis Reagent 1% SDS
SageHLS HLS-CATCH™ Kit cassettes and reagents to use on the SageHLS HMW Library System.
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