SageELF
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  • SageELF
  • SageELF
  • SageELF

SageELF Whole Sample Fractionator

Reference: ELF0001

The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA.

Features :

  • Reduces the complexity of RNA (cDNA) or protein samples
  • Allows construction of DNA libraries with multiple insert sizes for improved assembly
  • Allows sequencing of valuable DNA and retrieving sample for later study
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Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.

Pre-cast agarose gel cassettes for DNA fractionation

Gel cassettes for DNA fractionation are available in three agarose concentrations to provide a full range of options between 50 bp and 40 kb. For some applications, internal standards may be used for higher accuracy and reproducibility.

Gel cassette types and target ranges

  • 2% agarose, 100 bp – 2.2 kb
  • 0.75% agarose, 1 – 18 kb
  • 0.75% agarose, 10 – 40 kb

Pre-Cast SDS-agarose gel cassettes for protein fractionation

Gel cassettes for protein fractionation are available in two agarose concentrations to provide a full range of options for proteomic studies.

Gel cassette types and target ranges

  • 5% agarose, 10 - 100 kDa
  • 3% agarose, 50 – 500 kDa

The SageELF instrument includes a monitor, keyboard, and mouse. A CPU is built in -> an external PC is not required.

  • miRNA and RNA-seq
  • DNA sequencing
  • Mate-pair sequencing
  • Splice variant detection
  • Proteomics and mass spec
Electrophoresis voltage 25V to 150V constant, adjustable, or pulsed field
Optical detector 470 nm excitation | 525 nm emission
Power requirements 100-240 VAC, 2.5A, 50-60 Hz
Weight 9 kg
Dimensions (W x D x H) 34.0 x 27.0 x 27.0 cm
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