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CE IVD Multiplex Real-Time PCR Kits For Pathogen Detection

Reference: Mikrogen

Easy, Fast, Modular, Safe, Flexible and Compatible.

Developed with the clinical needs of the patient in mind, Mikrogen's range is based on the syndromic approach with all significant pathogens being detected for each disease category such as respiratory, gastrointestinal, sexually transmitted, and tick-borne infections.

Convince yourself of Mikrogen's high-quality CE IVD Multiplex real-time PCR product portfolio !

Features :

  • Complete PCR kits with ready-to-use reagents
  • Uniform cycling program for all pathogens
  • Toolbox System – combination of kits and panels according to individual needs
  • High sensitivity and specificity
  • High safety: universal internal control
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High flexibility and easy handling

  • Modular system: Standard cycling program for all ampliCube multiplex assays
  • Complete PCR kits with ready to use reagents
  • One positive control for all targets in one assay
  • Coloured (blue) enzyme mix for visualizing plate set up enables easy handling

Safe diagnosis

  • Full range of CE IVD kits
  • Excellent analytical performance; high sensitivity and specificity
  • Increased safety on reported results supporting also QM: internal control for monitoring RNA/DNA acid extraction and real time PCR inhibition in each primer & probe mix

Flexible and compatible

  • Compatible with most common real-time PCR cyclers
  • Compatible with different DNA/RNA extraction methods
  • Validated on Roche LightCycler® 480, Qiagen Rotor Gene®Q, Bio-Rad CFX96™, QuantStudio™5 Dx, Mic (Magnetic Induction Cycler from BMS)

Respiratory infections
Kit Name Description Cat#
ampliCube Respiratory Bacterial Panel 1 Real-time PCR multiplex kit for detection & semi-quantification of Chlamydia pneumoniae, Mycoplasma pneumoniae, Legionella spp. 50101
ampliCube Respiratory Bacterial Panel 1.1 Real-time PCR multiplex kit for detection & semi-quantification of Chlamydia pneumoniae, Mycoplasma pneumoniae, Legionella spp., Legionella pneumophila 50151
ampliCube Respiratory Bacterial Panel 2 Real-time PCR multiplex kit for detection of Bordetella pertussis, Bordetella parapertussis, Bordetella holmesii 50111
ampliCube Respiratory Bacterial Panel 3 Real-time PCR multiplex kit for detection & semi-quantification of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae 50121
ampliCube Respiratory Bacterial Panel 4
 Real-time PCR multiplex kit for detection & semi-quantification of Moraxella catarrhalis, Klebsiella pneumoniae/oxytoca 50131
ampliCube Respiratory Viral Panel 1 Real-time RT-PCR multiplex kit for detection of Influenza A, Influenza B, Influenza A H1N1 50102
ampliCube Respiratory Viral Panel 2 Real-time RT-PCR multiplex kit for detection of Parainfluenza 1-4, Bocavirus 50112
ampliCube Respiratory Viral Panel 3 Real-time RT-PCR multiplex kit for detection of Parechovirus, Metapneumovirus, RSV (A,B) 50122
ampliCube Respiratory Viral Panel 4
 Real-time RT-PCR multiplex kit for detection of Rhinovirus/Enterovirus, Adenovirus 50132
ampliCube Respiratory Viral Panel 5
 Real-time RT-PCR multiplex kit for detection of Influenza A, Influenza B, RSV (A, B) 50152
ampliCube Coronavirus Panel Real-time RT-PCR multiplex kit for detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV), SARS-CoV, hCoV Coronaviruses (NL63, OC43, 229E, HKU1) 50142
ampliCube Coronavirus SARS-CoV-2 Real-time RT-PCR multiplex kit for SARS Coronaviruses screening and SARS-CoV-2 confirmation 50143
ampliCube Flu & SARS-CoV-2 Real-time RT-PCR multiplex kit for Flu A, Flu B, SARS Coronaviruses screening and SARS-CoV-2 confirmation 50163 / 50173
alphaCube Bordetella Real-time PCR multiplex kit for detection of Bordetella pertussis, Bordetella parapertussis 830559
alphaCube Legionella Real-time PCR multiplex kit for detection of Legionella pneumophila 830539
alphaCube PanLegionella Real-time PCR multiplex kit for detection of Legionella spec. 830535
alphaCube MTB Real-time PCR multiplex kit for detection of Mycobacterium tuberculosis complex 830561
alphaCube MTB/NTM/MAC Real-time PCR multiplex kit for the detection of Mycobacterium tuberculosis complex (MTB), Non Tuberculous Mycobacteria (NTM) and Mycobacterium avium complex (MAC) 830566
alphaCube Entero 2.0 Real-time RT-PCR multiplex kit for detection of Enterovirus (Coxsackievirus A, Coxsackievirus B, Echovirus, numbered Enteroviruses, Polioviruses) 830509
alphaCube RespiraRNA 2.0 Real-time RT-PCR multiplex kit for detection of Influenza A and B, Respiratory Syncytial Virus (RSV) 830507

Gastrointestinal infections
Kit Name Description Cat#
ampliCube Gastrointestinal Bacterial Panel 1 Real-time PCR multiplex kit for detection of Campylobacter, Salmonella, Yersinia enterocolitica 50001
ampliCube Gastrointestinal Bacterial Panel 2 Real-time PCR multiplex kit for detection of VTEC (STX1+2), Shigella/EIEC and ETEC 50011
ampliCube Gastrointestinal Viral Panel 1 Real-time RT-PCR multiplex kit for detection of Norovirus (Genogroup I and II), Rotavirus, Adenovirus 50002
ampliCube Gastrointestinal Viral Panel 2 Real-time RT-PCR multiplex kit for detection of Sapovirus, Astrovirus 50012
alphaCube gastroplexVirus Real-time RT-PCR multiplex kit for detection of Norovirus (Genogroup I & II), Adenovirus, Rotavirus 830501
alphaCube gastroplexVirus PLUS 2.0 Real-time RT-PCR multiplex kit for detection of Norovirus Genogroup I, Norovirus Genogroup II, Adenovirus, Rotavirus, Astrovirus, Sapovirus 830503
alphaCube Entero 2.0
 Real-time RT-PCR multiplex kit for detection of Enterovirus (Coxsackievirus A, Coxsackievirus B, Echovirus, numbered Enteroviruses, Polioviruses) 830509
alphaCube Noro Real-time RT-PCR multiplex kit for detection of Norovirus (Genogroup I & II) 830513

Sexually transmitted diseases
Kit Name Description Cat#
ampliCube STD Panel 1 Real-time PCR multiplex kit for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium 50301
ampliCube STD Panel 2.1 Real-time PCR multiplex kit for detection of Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum 50322
ampliCube STD Panel 3
 Real-time PCR multiplex kit for detection of Herpes Simplex Virus (1, 2), Treponema pallidum 50303

Tick borne infections
Kit Name Description Cat#
alphaCube Babesia Real-time PCR multiplex kit for detection of Babesia divergens, Babesia microti and Babesia sp. EU1 830525
alphaCube Borrelia Real-time PCR multiplex kit for detection of Borrelia burgdorferi (sensu lato) 830527
alphaCube Borrelia/Rickettsia Real-time PCR multiplex kit for detection of Borrelia burgdorferi (sensu lato) and Rickettsia 830576
alphaCube Ehrlichia Real-time PCR multiplex kit for detection of E. chaffeensis, E. ewingii, Anaplasma phagocytophilum 830533
alphaCube Rickettsia Real-time PCR multiplex kit for detection of Rickettsia 830574
alphaCube TBE Real-time RT-PCR multiplex kit for detection of Tick Borne Encephalitis Virus 830519
alphaCube TBE/WNV Real-time PCR multiplex kit for detection of Tick Borne Encephalitis Virus, West Nile Virus 830568
alphaCube Q-Fever Real-time PCR multiplex kit for detection of Coxiella burnetii 830555

Hepatitis
Kit Name Description Cat#
ampliCube HEV 2.0 Quant Real-time RT-PCR multiplex kit for detection & quantification of HEV (Hepatitis E Virus) 55002

Tropical fever
Kit Name Description Cat#
ampliCube Zika Virus Real-time RT-PCR multiplex kit for detection of Zika Virus (ZIKV) 57801
alphaCube TBE/WNV Real-time PCR multiplex kit for detection of Tick Borne Encephalitis Virus, West Nile Virus 830568
alphaCube Tropical Fever 1 Real-time RT-PCR multiplex kit for detection of Zika Virus, Chikungunya Virus, Dengue Viruses 1-4 and Yellow Fever Virus 830563

Miscellaneous
Kit Name Description Cat#
alphaCube GBS Real-time PCR multiplex kit for detection of Streptococcus agalactiae (Group B Streptococcus (GBS)) 830547
alphaCube HSV/VZV Real-time PCR multiplex kit for detection of Herpes Simplex Virus (HSV-1, HSV-2), Varicella Zoster Virus 830572
alphaCube Monkeypox virus (RUO)  Reagents to detect the DNA of Monkeypox virus (OPG185 gene) 830581
alphaCube MRSA 3.0 Real-time PCR multiplex kit for detection of methicillin-resistant Staphylococcus aureus (MRSA). The PCR targets are the orfX/SSCmec junction and the mecA/mecC genes 830557
alphaCube PVL-MRSA Real-time PCR multiplex kit for detection of methicillin-resistant S. aureus (MRSA), methicillin sensitive S. aureus (MSSA), methicillin-resistant coagulase-negative S. (MRConS), Community-aquired MRSA (PVL toxin, pathogenic factor) 830570

Multiple drug resistance
Kit Name Description Cat#
ampliCube MDR Panel 1 Real-time PCR multiplex kit for detection of NDM (New Delhi metallo-ß-lactamase), KPC (Klebsiella pneumoniae carbapenemase), GIM (German Imipenemase) resistance genes 50401
ampliCube MDR Panel 2 Real-time PCR multiplex kit for detection of NDM (New Delhi metallo-ß-lactamase), KPC (Klebsiella pneumoniae carbapenemase), SPM (Sao Paolo metallo-ß-lactamase) resistance genes 50402
ampliCube MDR Panel 3 Real-time PCR multiplex kit for detection of CTX-M15 Cefotaximase-Munich (group 1), CTX-M14 Cefotaximase-Munich (group 9) resistance genes 50403
ampliCube MDR Panel 4 Real-time PCR multiplex kit for detection of IMP (Imipenemase), VIM (Verona Integron-encoded metallo-ß-lactamase), OXA (Oxacillinase OXA-48-like) resistance genes 50404
ampliCube MDR Panel 5 Real-time PCR multiplex kit for detection of NDM (New Delhi metallo-ß-lactamase), OXA (Oxacillinase OXA-23-like, OXA-24/40-like, OXA-58-like), OXA (Oxacillinase ISAba1/OXA-51-like) resistance genes 50405
ampliCube MDR Panel 6 Real-time PCR multiplex kit for detection of cfrA/B (Chloramphenicol Florfenicol resistance), optrA (Oxazolidinonephenicol resistance), vanA (Vancomycin A-type resistance), vanB (Vancomycin B-type resistance) resistance genes 50406

Sample purification

Works with a large range of automated and manual extraction kits. Contact us for more details.

Compatible Real-Time PCR instruments

  • Applied Biosystems® QuantStudio™5 Dx, 7500/7500 Fast
  • Qiagen Rotor-Gene Q
  • Corbett Rotor-Gene 6000
  • Bio-Rad CFX96™
  • Roche LightCycler® 480 II
  • Mic Real-Time PCR System (Bio Molecular Systems)
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