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SensiFAST HRM Kit

Reference: BIO32005

SensiFAST™ HRM uses the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis.

Features :

  • Ultra-sensitive: accurately discriminates class 4 (A/T) SNP mutations
  • Rapid - enabling accurate results to be achieved faster
  • Highly reproducible - highly-specific, high-resolution melt analysis
  • Optimized - assays quickly and reliably established, even for difficult genomic loci

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CHF 510.00
Tax excluded
Packaging: 500 rxns (20 µl)
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High Resolution Melt (HRM) curve analysis is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature. It uses intercalating dyes, highly accurate melt curves and the application of specific statistical analyses.

SensiFAST HRM has been developed using the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis, ideal for small and medium-sized genotyping projects.

The combination of our SensiFAST DNA Polymerase, buffer and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences, such as class 4 SNPs without sequence preference.

Instrument Compatibility

ABI 7300, 7500 FAST, Qiagen Rotor-Gene™ 6000, Eppendorf Mastercycler® ep realplex, Illumina® Eco™, Roche LightCycler®; 96 and 480, Bio-Rad Opticon®, CFX96™, CFX384™, Idaho LightScanner® 32 (LS32™), Takara Thermal Cycler Dice (TP800) (see Specifications tab for product selection table).

  • Single Nucleotide Polymorphisms (SNPs) genotyping
  • Epigenetics (DNA methylation analysis)
  • Zygosity testing (DNA mapping and DNA fingerprinting)
  • Gene scanning (search for the presence of unknown variations in PCR amplicons)

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