MyFi DNA Polymerase

MyFi DNA Polymerase is a high-performance, new generation DNA polymerase complex, combining the sensitivity of our ever-popular MyTaq HS DNA Polymerase with a highly processive proof-reading DNA polymerase. It has been developed to give reliable amplification of targets up to 10 kb from challenging and complex targets, even in the presence of PCR inhibitors. The pre-optimized MyFi Mix formulation, supplied in a convenient single tube, reduces the number of pipetting steps improving throughput and reproducibility.

MyFi Mix has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl₂ and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates. MyFi Mix generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi Mix provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.