MyFi Mix

MyFi Mix is a ready-to-use 2X mix formulation, containing all the components (including proprietary enhancers and stabilizers) necessary for trouble-free PCR reaction assembly, while delivering the same unique balance of PCR sensitivity and high-fidelity. It has been developed to give reliable amplification of targets up to 10 kb from challenging and complex targets, even in the presence of PCR inhibitors. The pre-optimized MyFi Mix formulation, supplied in a convenient single tube, reduces the number of pipetting steps improving throughput and reproducibility.

MyFi Mix has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl₂ and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates. MyFi Mix generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi Mix provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.