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ACCUZYME Mix (High Fidelity PCR)

Reference: BIO25028

ACCUZYME Mix contains a robust, efficient proofreading enzyme that gives increased fidelity in high-yield PCR, for use in all routine cloning applications.

Features :

  • Efficient – highly productive target amplification and removal of 3’ A overhangs
  • Accurate – possesses 3’ - 5’ proofreading exonuclease activity
  • Sensitive – high-yield amplification from limiting amounts of template DNA
  • Robust – developed for reliable amplification of even the most challenging targets
  • Convenient – advanced buffering system minimizes the requirements for PCR optimization
  • Simple – mix reduces pipetting burden for increased consistency in assay set-up

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CHF 929.00
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Packaging: 500 rxns (50 µl)
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ACCUZYME is recommended for all routine cloning applications. ACCUZYME is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME has an error-rate of 3.0 x 10-6, and results in blunt-ended amplicons of up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis.

ACCUZYME is supplied with a buffering system that provides ideal conditions for most assays. Consequently, the cost and effort typically associated with optimizing assay performance is often eliminated.  Nevertheless, in circumstances where further optimization is required to improve PCR specificity and/or yield, ACCUZYME includes an additional vial of MgCl2. ACCUZYME Mix dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured by the reduction in the number of pipetting steps that can lead to pipetting errors.

  • Cloning techniques where high fidelity is desirable and requiring increased PCR yield
  • GC-rich templates
  • Blunt-end cloning
  • Site directed mutagenesis

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