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CleanNGS DNA & RNA Cleanup For NGS Library Construction

Reference: CNGS0050

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The CleanNGS kit offers a highly efficient magnetic bead based cleanup system for the purification of both DNA and/or RNA for Next-Generation Sequencing workflows.

Features :

  • Designed for both DNA and RNA purification
  • Ideal for (double-sided) size selection for Next-Generation Sequencing
  • Efficient removal of unincorporated dNTPs, primers, primer dimers and other contaminants
  • No centrifugation or filtration

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CleanNGS provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).
Based on our proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications such. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.

Samples purified with CleanNGS system are ready to be used in the following applications :

  • Next-Generation Sequencing
  • PCR
  • Genomic DNA cleanup
  • RNA cleanup
  • Fragment analysis
  • Microarrays
  • Restriction enzyme cleanup
  • Cloning
Product number Description Number of reactions
 Storage conditions
CNGS0050D CleanNGS – 50 ml 2,777 * 4-8⁰C
DO NOT FREEZE
CNGS0500D CleanNGS – 500 ml 27,777 *
* Number of reactions is based on a typical 10 μl PCR reaction volume. Volume of CleanNGS to be used per reaction = 1.8X the sample volume.

Brochure

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User Manual

Download (319.6KB)

App Note NGS Lib Purification

Download (328.89KB)

App Note NGS Lib Size Selection

Download (303.92KB)

Performance

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