Clean FFPE DNA & RNA Purification Kit

The Clean FFPE DNA & RNA Kit is designed for both DNA and RNA isolation from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. During the isolation process, the DNA and RNA will be isolated and purified from the same sample. This process provides a more comprehensive analysis from precious FFPE samples.

The protocol utilizes a specially formulated buffer system that not only partially reverses the formaldehyde-induced crosslinking but also ensures DNA and RNA are differentially purified with no cross-contamination. Using a specially formulated lysis buffer, samples are lysed and the DNA is bound to the CleanNA magnetic particles. The supernatant can then be removed and processed separately for RNA isolation.
Magnetic beads are separated from solution using a magnetic separation device. Following a few rapid wash steps trace contaminants are removed. The purified DNA and/or RNA are then eluted from the CleanNA particles using an elution buffer or nuclease free water. After completing the protocol, the isolated and purified DNA and RNA samples are directly suitable for a variety of downstream applications including SNP analysis, next generation sequencing, and genotyping.

The kit's protocol is fully scalable and due to the use of CleanNA magnetic bead purification technology, can besides manual usage, easily be automated once the samples have been lysed on liquid handling workstations.