Naica Nio+ 7-color Digital PCR

Automation-friendly

The same dimensions as the lab consumables you already use, and compatible with your existing equipment. Our Ruby Chip is the most user-friendly microfluidics consumable we have built to date.

Capacity of 768 chambers

Nio+ supports a high-throughput capacity of 768 chambers processed per 8-hour workday. Below are examples of how that capacity can be reached, and the flexibility of Nio+.

Continuous loading

Nio+ is the only digital PCR instrument that works like labs work. With the capacity to handle samples incoming at changing intervals. Anytime you are ready, for up to 8 Chip Plates, Nio+ assumes the tempo your lab operates at.

All-in-one

Nio+ is the smallest all-in-one digital PCR instrument at its capacity. With a footprint of just 56 cm by 67 cm, it fits in even the smallest lab.

7 colors

Nio+ is the only instrument that allows for the reading of 7 colors. State of the art imaging system, with support for the colors and dyes you use today :

Quantify 4x more targets in a single assay using color combination

In simple color multiplexing, a target is detected using a single fluorophore in a single channel. In color combination multiplexing, a target is detected using two separate fluorophores detected in 2 different channels. Such a strategy allows the development of assays to detect and individually quantify much more targets using Nio+. Thanks to a population editor based on fluorescence co-localization criteria, our software seamlessly integrates the analysis of color combination assays. A 16-target example :

Cost effective high-plex assay design with Stilla^®’s Crystal Flex Probe technology

Stilla^® developed a modular detection chemistry that allows for flexible, fast and cost-efficient assay design. Introducing Crystal Flex Probes and Crystal Universal Reporters, assay design for high multiplexed panels in Crystal Digital PCR^®. By incorporating the fluorophore into the Crystal Universal Reporters, Stilla's Crystal Flex Probe technology allows the use of the same Crystal Universal Reporters across all assays. This means that only the non-fluorescent probes (Crystal Flex Probes) need to be customized to your specific target. Resulting in faster, simpler and cheaper multiplexed digital PCR assays.

• Cost effective - Reduce cost by using the same set of fluorescent reporters to detect targets accross different panels
• Time saving - Accelerate assay design by relying on non-fluorescent oligos for assay customization and optimization

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A powerful analysis software

Crystal Miner software automatically identifies positive and negative droplets in all the fluorescence channels of interest and measures the concentrations of targeted nucleic acids.

With its intuitive visuals, Crystal Miner software eases the analysis of complex and highly multiplexed assays.

The unique Explore Crystal feature linking any dot in a dot plot to its corresponding droplet in the Droplet Crystal allows for a comprehensive analysis of the dot plots and provides precious information on assay performances for a better control of the quality of the experiment.

Along with the ability to read data arranged along 1D, 2D, and 3D dot plots, it is possible to get access to raw data and export experimental details and results.